Volume 29, Issue 4, 2020
DOI: 10.24205/03276716.2020.810
Expression of GNAS-AS1 In Rectal Cancer and Its Effects on Proliferation, Migration and Invasion of Rectal Cancer Cells
Abstract
Objective: To study the molecular mechanism of long non-coding ribonucleic acid (lncRNA) GNAS antisense RNA 1 (GNAS-AS1) in affecting the proliferation, migration and invasion of rectal cancer cells.
Methods: The levels of GNAS-AS1 and micro ribonucleic acid (miR)-34c-5p in rectal cancer tissues, paracancerous tissues and rectal cancer SW1463 cells were determined using qRT-PCR, and Western blotting was conducted to measure the content of p21, E-cadherin and matrix metalloproteinase (MMP)-2 proteins. The cell viability rate was measured using MTT assay, and the number of migrating and invading cells was counted by Transwell assay. The targeting relationship between GNAS-AS1 and miR-34c-5p was verified using dual luciferase reporter assay.
Results: Compared with normal paracancerous tissues, rectal cancer tissues had significantly increased GNSA-AS1 content (P<0.05). Inhibiting the expression of GNAS-AS1 suppressed the proliferation, migration and invasion of rectal cancer SW1463 cells, raised the content of p21 and E-cadherin proteins and the percentage of cells in G0 phase and decreased the content of MMP-2 and the percentage of cells in S1 phase. GNAS-AS1 bound to miR-34c-5p in a targeted manner, and their expressions showed a negative correlation. Moreover, repressing the expression of miR-34c-5p weakened the effects of down-regulated GNAS-AS1 expression on the proliferation, migration and invasion of SW1463 cells.
Conclusion: GNAS-AS1 modulates the proliferation, migration and invasion of rectal cancer SW1463 cells by targeting miR-34c-5p.
Keywords
rectal cancer, GNAS-AS1, miR-34c-5p, proliferation, migration, invasion.