Volume 30, Issue 2, 2021
DOI: 10.24205/03276716.2020.4035
Correlation of Toll-like Receptor Single Nucleotide Polymorphisms with Susceptibility to Infectious ARDS in Children
Abstract
Purpose: Acute respiratory distress syndrome (ARDS) is a major contributing factor to death in child intensive care units. Toll-like receptor (TLR) single nucleotide polymorphisms (SNPs) participate in several diseases. This study intends to analyze the correlation between TLR2 and TLR4 SNPs and the susceptibility to infectious ARDS in children.
Materials and Method: 32 patients with infectious ARDS diagnosed in our hospital were collected and 35 healthy children were collected as the control group followed by analysis of TLR2 and TLR4 mRNA expression in PBMCs by real time PCR. The SNaPshot method was used to detect TLR2 (rs5743708, rs3804099) and TLR4 genes (rs10759932, rs4986790) genotypes. Their correlation with disease susceptibility was analyzed.
Results: TLR2 and TLR4 level was significantly upregulated in PBMCs of children with infectious ARDS compared to control. The genotypes of TLR2 gene rs5743708 and TLR4 gene rs4986790 were positively correlated with BMI (P <0.05) but not with age and gender. There was no significant difference in the frequency composition ratios of rs3804099 and rs10759932 genotypes, which was not related to ARDS. TLR2 gene rs5743708 and TLR4 gene rs4986790 genotype distribution showed a significant difference between two groups (P <0.05) and they were related to ARDS, and their mutant alleles increased the risk of ARDS (OR 1.92, 95% CI1 .27-5.91; OR 1.88, 95% CI 1.32-6.76) (P <0.05).
Conclusions: TLR2 and TLR4 level is upregulated in children with infectious ARDS. TLR2 rs5743708 and TLR4rs4986790 may be ARDS susceptible genes
Keywords
infectious ARDS; TLR2; TLR4; gene; polymorphism; SNP