Volume 32, Issue 1, 2023
DOI: 10.53555/03276716.2023.7007
Green Carbon Dots Derived From Acetosa Sagittata Aerial Parts And In Vitro Antidiabetic, Antioxidant And Cytotoxic Properties
Abstract
Diabetes mellitus is one of the leading causes of mortality and morbidity worldwide. Several conventional drugs are used to treat hyperglycemic condition in DM but associated with side effects. Carbon dots are alternative small molecules and able to inhibit enzyme activity through their surface functional groups. Green carbon dots are low toxic and biocompatible in nature which are synthesized using renewable precursors. In the present study, the carbon dots were synthesized using hydrothermal assisted method and physiochemical and morphological properties were confirmed using UV-Vis, FTIR, Fluorescence spectroscopy, XRD and HRTEM. Among all three in vitro antioxidant activities DPPH and FRAP assays revealed the efficient free radical neutralizing capability of CDs than ABTS assay with IC50 ranges between 74 to 526 µg mL-1. The CDs exhibited effective ?-amylase inhibitory activity than ?-glucosidase inhibitory activity with lowest IC50 recorded for CD1 sample against ?-amylase (61.25 µg mL-1). For cytotoxicity CD1 shown lowest IC50 value against HeLa cell lines (84.44 µg mL-1). Hence the CDs are effective in inhibiting the enzyme activity and neutralizing the free radicals
Keywords
Carbon dots, Acetosa sagittata, Anti-diabetic, Antioxidant, Cytotoxicity